Title
Graphite nanoparticle as nanoquecher for 17β-estradiol detection using shortening aptamer sequence
Author
Xiaoli Qi, Hui Hu, Yuesuo Yang, Yunxian Piao
Year
2018
Journal
Analyst
Abstract
Graphite nanoparticle (GN) with spherical shape and stacked by a few layered graphene sheets is an ideal fluorescent nanoquencher for the fluorophore. We developed a novel aptasensor based on fluorescence resonance energy transfer for sensitive and specific detection of 17β-estradiol (E2) by using the GN as fluorescent nanoquencher and the shorten E2 specific aptamer as sensing probe. The physiological and chemical properties of the aptasensor in responses to the E2 capturing were investigated with atomic force microscopic analysis and E2 detection principle was clarified accordingly. Also it was demonstrated the sensitivity of the aptasensor was affected by the length of aptamers and the particle size of the nanoquenchers, and highest sensitivity for E2 detection was achieved with the shorter aptamer of 35 base sequences and the smaller GN with particle size of around 5 nm. And the detection limit was 1.02 ng/mL. Moreover, it presented no cross reaction with E2 analogs and was successfully utilized for the real environmental water monitoring.
Instrument
J-810
Keywords
Circular dichroism, DNA structure, Nanostructures, Sensors, Materials, Biochemistry