A colorimetric sensor for hydrogen sulfide detection using direct inhibition of active site in G-quadruplex DNAzyme
Seungyoon Kang, Jinyoung Oh, Min Su Han
Dyes and Pigments
Because of the toxicity of hydrogen sulfide and its important role in various physiological processes, the development of a selective and sensitive hydrogen sulfide sensing method is vital. In this study, a colorimetric method for hydrogen sulfide detection was developed by exploiting the direct inhibition of active site in G-quadruplex DNAzyme by hydrogen sulfide. Hydrogen sulfide inactivates the peroxidase-like activity of PS2.M-hemin G-quadruplex DNAzyme by directly blocking the active site of the DNAzyme, allowing the label-free colorimetric detection of hydrogen sulfide by the naked eye by using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS2−) as a peroxidase substrate. From titration results, the detection limit of this colorimetric hydrogen sulfide sensor was estimated to be 410 nM. In addition, the sensor is highly selective for hydrogen sulfide over various anions and was used to determine hydrogen sulfide concentrations in tap water samples.
Circular dichroism, DNA structure, Ligand binding, Biochemistry, Materials