Title
A simple design of fluorescent probe for indirect detection of β-lactamase based on AIE and ESIPT processes
Author
Lu Peng, Lu Xiao, Yiwen Ding, Yu Xiang, Aijun Tong
Year
2018
Journal
Journal of Materials Chemistry B
Abstract
A novel fluorescent probe DNBS-CSA is developed for light-up detection of β-lactamase. The probe design is based on an indirect detection approach with three step reactions. β-Lactamase can react with the lactam of its substrate (cefazolin sodium) to produce a secondary amine, initiating a spontaneous elimination reaction and affording a thiol compound. The thiol could further react with the sulfonate group of DNBS-CSA, releasing the salicylaldehyde azine derivative (CSA) with both aggregation induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) characteristics. Previously reported β-lactamase probes require covalent linkage of the substrate β-lactam ring part to probe, which makes the probe synthesis difficult due to the complicated structure of β-lactam ring. On the contrary, modification of β-lactam ring is no longer necessary for DNBS-CSA according to our indirect detection approach. The linear range of fluorescent quantification for β-lactamase is 0-10 mU/mL in aqueous solution. Moreover, owing to the AIE property of CSA, detection of β-lactamase with DNBS-CSA on test papers was also achieved.
Instrument
FP-8600
Keywords
Fluorescence, Chemical stability, Aggregation, Sensors, Kinetics, Materials, Biochemistry