Antimicrobial Activity of Chimera Peptides Composed of Human Neutrophil Peptide 1 (HNP-1) Truncated Analogues and Bovine Lactoferrampin
Natalia Ptaszyńska, Katarzyna Gucwa, Anna Łęgowska, Dawid Dębowski, Agata Gitlin-Domagalska, Jan Lica, Mateusz Heldt, Dorota Martynow, Mateusz Olszewski, Sławomir Milewski, Tzi Bun Ng, Krzysztof Rolka
Three chimera peptides composed of bovine lactoferrampin and the analogue of truncated human neutrophil peptide 1 were synthesized by the solid-phase method. In two compounds peptide chains were connected via isopeptide bond, whereas in the third one disulfide bridge served as a linker. All three chimeras displayed significantly higher antimicrobial activity than the constituent peptides as well as their equimolar mixtures. The one with a disulfide bridge displayed selectivity toward Gram-positive bacteria and was able to penetrate bacterial cells. The chimeric peptides demonstrated low in vitro mammalian cytotoxicity, especially against benign cells. The significance of linker type was also reflected in the secondary structure and proteolytic stability of studied compounds. Presented results proved that such chimeras are good lead structures for designing antimicrobial drugs.
Circular dichroism, Secondary structure, Chemical stability, Biochemistry