Title
Collagen-ZnO scaffolds for wound healing applications: Role of dendrimer functionalization and nanoparticle morphology
Author
Mohan Vedhanayagam, Balachandran Unni Nair, Kalarical Janardhanan Sreeram
Year
2018
Journal
ACS Applied Biomaterials
Abstract
Functionalized nanoparticle cross-linked collagen scaffolds offer improved properties to biomaterials and regenerated tissues. As influence of nanoparticle shape on collagen scaffold has received little attention. The present study, evaluates the role of ZnO nanoparticle shape (Sphere, Rod, Hexagonal, Needle, Flower, Star and Circlar disk, Dough nut and Cube on collagen self-assembly. Nanoparticle was prepared by using co-precipitation method and subsequently functionalized with Triethoxysilane poly(amidoamine) dendrimer generation 1 (TES-PAMAM-G1) on nanoparticle surface. The self-assembly process of collagen, facilitated by EDC-NHS cross-linking led to stable ZnO-TES-PAMAM-G1-collagen scaffolds. Physicochemical properties and biocompatibility of scaffolds were analysed for the functional groups present, morphology, thermal transformation and cell viability etc and obtained results compared against collagen scaffolds with/without EDC-NHS crosslinking. In vivo wound healing activity of ZnO-TES-PAMAM-G1-collagen scaffolds were tested on Albino rats that were subjected to excisional wounds and results were compared with control and collagen scaffold. Our findings suggested that the functionalized nanostructure mediated collagen scaffolds exhibited higher thermal (92oC 0.3), mechanical stability (130.23-305.45 MPa 0.1-2.0) than collagen scaffold (77.36 0.5 oC and 7.96 MPa 0.8). The result of in-vivo wound healing study indicate that spherical shape of ZnO-TES-PAMAM -G1 NPs cross-linked collagen scaffold showed enhanced re-epithelization and faster collagen deposition than other scaffolds probably owing to their higher surface area, leading to higher grafting density on the surface. This work provides a new approach for designing nanoparticle mediated collagen scaffold for wound healing application.
Instrument
J-815
Keywords
Circular dichroism, Secondary structure, Nanostructures, Materials, Biochemistry