Title
Comparative effect of cationic gemini surfactant and its monomeric counterpart on the conformational stability of phospholipase A2
Author
Mehrajud din Parray, Neha Maurya, Farooq Ahmad Wani, Mahendra S. Borse, Najmul Arfin, Maqsood Ahmad Malik, Rajan Patel
Year
2018
Journal
Journal of Molecular Structure
Abstract
Herein, we have investigated the effect of cationic gemini surfactant (hexanediyl-α,ω-bis-(N-(2-hydroxyethyl)N-methylhexadecylammonium bromide), (16-6-16MEA) and its monomeric counterpart (N-(2-hyroxyethyl)N, N-dimethylhexadecylammonium bromide), (16MEA) on the conformational stability of phospholipase A2 (PLA2). The interaction of gemini surfactant and its monomeric counterpart with PLA2 was characterized by utilizing steady-state fluorescence, time-resolved fluorescence, CD spectroscopy, and computational methods. The steady-state fluorescence results suggested the involvement of static quenching mechanism which was further supported by time resolved fluorescence measurements. The Stern-Volmer equation was utilized to calculate the value of Stern-Volmer quenching constant KSV. The stoichiometric binding ratio of PLA2 with 16MEA and 16-6-16MEA was observed to be 1:1. The far-UV CD spectra for PLA2 revealed similar alteration in secondary structure, in presence of 16MEA and 16-6-16 MEA, which was further supported by computational methods.
Instrument
J-1500
Keywords
Circular dichroism, Secondary structure, Chemical stability, Biochemistry