Title
Controlled rate slow freezing with lyoprotective agent to retain the integrity of lipid nanovesicles during lyophilization
Author
Eunhye Yang, Hyunjiong Yu, SungHak Choi, Kyung-Min Park, Ho-Sup-Jung & Pahn-Shick Chang
Year
2021
Journal
Scientific Reports
Abstract
We designed a novel lyophilization method using controlled rate slow freezing (CSF) with lyoprotective agent (LPA) to achieve intact lipid nanovesicles after lyophilization. During the freezing step, LPA prevented water supercooling, and the freezing rate was controlled by CSF. Regulating the freezing rate by various liquid media was a crucial determinant of membrane disruption, and isopropanol (freezing rate of 0.933 °C/min) was the optimal medium for the CSF system. Lyophilized lipid nanovesicle using both CSF and LPA retained 92.9% of the core material and had uniform size distributions (Z-average diameter = 133.4 nm, polydispersity index = 0.144), similar to intact vesicles (120.7 nm and 0.159, respectively), after rehydration. Only lyophilized lipid nanovesicle using both CSF and LPA showed no changes in membrane fluidity and polarity. This lyophilization method can be applied to improve storage stability of lipid nanocarriers encapsulating drugs while retaining their original activity.
Full Article
Instrument
FP-8500
Keywords
CSF, LPA, nanocarriers