Title
Curcumin encapsulated in the complex of lysozyme/carboxymethylcellulose and implications for the antioxidant activity of curcumin
Author
Zhenshun Li, Yuntao Wang, Yaqiong Pei, Wenfei Xiong, Chunlan Zhang, Wei Xu, Shilin Liu, Bin Li
Year
2015
Journal
Food Research International
Abstract
A facile approach was investigated to encapsulate and protect curcumin (Cur) by self-assembly of lysozyme (Ly) and carboxymethylcellulose (CMC) of different degrees of substitution (DSs). This work studied the influence of Ly–CMC coacervates on the binding, solubility and stability of Cur. The interactions of Cur with Ly–CMC coacervates were researched by UV–vis, steady-state fluorescence, synchronous fluorescence and circular dichroism spectra. These results were explained in terms of the formation of Ly–CMC coacervates with electrostatic interaction, which led to unfold the structure of Ly for providing Cur with more hydrophobic microenvironment than free Ly. Meanwhile, the CMC of higher DS provide more negative charges, and produce more attraction to Ly than that of lower DS values. Moreover, the coacervates of Ly–CMC of higher DS are found to be superior for enhancing the stability of Cur. Our work provided a new insight for understanding the biomolecule protective system based on protein/polysaccharide complexes.
Instrument
J-1500
Keywords
Circular dichroism, Protein folding, Ligand binding, Food science, Biochemistry