Title
Detection of Reactive Oxygen Species (ROS) by a Carbon-Dot-Ascorbic Acid Hydrogel
Author
Sagarika Bhattacharya, Rhitajit Sarkar, Sukhendu Nandi, Angel Porgador, Raz Jelinek
Year
2016
Journal
Analytical Chemistry
Abstract
Detection of reactive oxygen species (ROS) is important in varied biological processes, disease diagnostics, and chemotherapeutic drug screening. We constructed a ROS sensor comprising an ascorbic-acid-based hydrogel encapsulating lumi-nescent amphiphilic carbon dots (C-dots). The sensing mechanism is based upon ROS-induced oxidation of the ascorbic acid units within the hydrogel scaffold; as a consequence, the hydrogel framework collapses resulting in aggregation of the C-dots and quenching of their luminescence. The C-dot-hydrogel platform exhibits high sensitivity and detected ROS generated chemically in solution and in actual cell environments. We demonstrate application of the C-dot-hydrogel for evaluating efficacy of a chemo-therapeutic substance, underscoring the potential of the system for drug screening applications.
Instrument
J-715
Keywords
Circular dichroism, Secondary structure, Biochemistry, Materials