Title
Development of fluorescence oligonucleotide probes based on cytosine- and guanine-rich sequences
Author
Anna Dembska, Angelika Świtalska, Agnieszka Fedoruk-Wyszomirska, Bernard Juskowiak
Year
2020
Journal
Scientific Reports
Abstract
The properties of cytosine- and guanine-rich oligonucleotides contributed to employing them as sensing elements in various biosensors. In this paper, we report our current development of fluorescence oligonucleotide probes based on i-motif or G-quadruplex forming oligonucleotides for cellular measurements or bioimaging applications. Additionally, we also focus on the spectral properties of the new fluorescent silver nanoclusters based system (ChONC12-AgNCs) that is able to anchor at the Langmuir monolayer interface, which is mimicking the surface of living cells membrane.
Full Article
Instrument
V-750, FP-8200, J-820
Keywords
Circular dichroism, DNA structure, Chemical stability, Absorption, Fluorescence, G-quadruplex structure, Nanostructures, Materials, Biochemistry