Title
Discovery of Neolignan Glycosides with Acetylcolinesterase Inhibitory Activity from Huangjinya Green Tea Guided by Ultra Performance Liquid Chromatography–Tandem Mass Spectrometry Data and Global Natural Product Social Molecular Networking
Author
Hao-Yue Wu, Jia-Ping Ke, Wei Wang, Ya-Shuai Kong, Peng Zhang, Tie-Jun Ling, Guan-Hu Bao
Year
2019
Journal
Journal of Agricultural and Food Chemistry
Abstract
Global Natural Product Social feature-based networking was applied to follow the phytochemicals, including nine flavonoid glycosides, six catechins, and three flavonols in Huangjinya green tea. Further, a new 8-O-4′-type neolignan glycoside, camellignanoside A (1), and 15 known compounds (2–16) were isolated through a variety of column chromatographies, and the structure was elucidated extensively by ultra performance liquid chromatography–quadrupole–time-of-flight–tandem mass spectrometry, 1H and 13C nuclear magnetic resonance, heteronuclear single-quantum correlation, heteronuclear multiple-bond correlation, 1H–1H correlation spectroscopy, rotating frame nuclear Overhauser effect spectroscopy, and Nuclear Overhauser effect spectroscopy, and circular dichroism spectroscopies. Compounds 1 and 2 showed acetylcolinesterase inhibition activity, with IC50 = 0.75 and 0.18 μM, respectively.
Instrument
J-815
Keywords
Circular dichroism, Absolute configuration, Natural products, Agricultural and environmental