Discovery of Neolignan Glycosides with Acetylcolinesterase Inhibitory Activity from Huangjinya Green Tea Guided by Ultra Performance Liquid Chromatography–Tandem Mass Spectrometry Data and Global Natural Product Social Molecular Networking
Hao-Yue Wu, Jia-Ping Ke, Wei Wang, Ya-Shuai Kong, Peng Zhang, Tie-Jun Ling, Guan-Hu Bao
Journal of Agricultural and Food Chemistry
Global Natural Product Social feature-based networking was applied to follow the phytochemicals, including nine flavonoid glycosides, six catechins, and three flavonols in Huangjinya green tea. Further, a new 8-O-4′-type neolignan glycoside, camellignanoside A (1), and 15 known compounds (2–16) were isolated through a variety of column chromatographies, and the structure was elucidated extensively by ultra performance liquid chromatography–quadrupole–time-of-flight–tandem mass spectrometry, 1H and 13C nuclear magnetic resonance, heteronuclear single-quantum correlation, heteronuclear multiple-bond correlation, 1H–1H correlation spectroscopy, rotating frame nuclear Overhauser effect spectroscopy, and Nuclear Overhauser effect spectroscopy, and circular dichroism spectroscopies. Compounds 1 and 2 showed acetylcolinesterase inhibition activity, with IC50 = 0.75 and 0.18 μM, respectively.
Circular dichroism, Absolute configuration, Natural products, Agricultural and environmental