Engineered Protein Polymer-Gold Nanoparticle Hybrid Materials for Small Molecule Delivery
Min Dal, JA Frezzo, E Sharma, R Chen, N Singh, C Yuvienco, E Caglar, S Xiao,2 A Saxena, JK Montclare
Journal of Nanomedicine and Nanotechnology
We have fabricated protein polymer-gold nanoparticle (P-GNP) nanocomposites that exhibit enhanced binding and delivery properties of the small hydrophobic molecule drug, curcumin, to the model breast cancer cell line, MCF-7. These hybrid biomaterials are constructed via in situ GNP templated-synthesis with genetically engineered histidine tags. The P-GNP nanocomposites exhibit enhanced small molecule loading, sustained release and increased uptake by MCF-7 cells. When compared to the proteins polymers alone, the P-GNPs demonstrate a greater than 7-fold increase in curcumin binding, a nearly 50% slower release profile and more than 2-fold increase in cellular uptake of curcumin. These results suggest that P-GNP nanocomposites serve as promising candidates for drug delivery vehicles.
Circular dichroism, Nanostructures, Polymers, Secondary structure, Thermal stability, Pharmaceutical, Biochemistry, Materials