Exploration of a multi-target ligand, dehydroevodiamine, for the recognition of three G-quadruplexes in c-Myb proto-oncogene by ESI–MS

July 28, 2017

Title

Exploration of a multi-target ligand, dehydroevodiamine, for the recognition of three G-quadruplexes in c-Myb proto-oncogene by ESI–MS

Author

Fangyuan Li, Jiang Zhou, Ming Xu, Gu Yuan

Year

2017

Journal

International Journal of Mass Spectrometry

Abstract

In this research, ESI mass spectrometry was used to probe the formation of G-quadruplexes from three G-rich sequences (S1–S3) in the upstream region of the transcription start site in human c-Myb proto-oncogene. A ligand, dehydroevodiamine, was found for its high binding affinities towards the c-Myb G-quadruplexes. In addition, dehydroevodiamine bound towards the Q1–Q3 with high selectivity over long-chain duplex DNA. High-resolution ESI–MS was utilized to investigate the binding competition of dehydroevodiamine in the mixture solution of Q1–Q3 G-quadruplexes, and it appeared to have the binding affinity in the following order: Q3 ≈ Q1 > Q2, which is consistent with the results in the single G-quadruplex solutions. The properties of dehydroevodiamine gave its potential in the future studies of c-Myb expression regulation.

Instrument

J-815

Keywords

Circular dichroism, DNA structure, Chemical stability, Thermal stability, Thermodynamics, Biochemistry