Title
High-resolution DNA quadruplex structure containing all the A-, G-, C-, T-tetrads
Author
Hehua Liu, Rui Wang, Xiang Yu, Fusheng Shen, Wenxian Lan, Phensinee Haruehanroengra, Qingqing Yao, Jing Zhang, Yiqing Chen, Suhua Li, Baixing Wu, Lina Zheng, Jinbiao Ma, Jinzhong Lin, Chunyang Cao, Jixi Li, Jia Sheng, Jianhua Gan
Year
2018
Journal
Nucleic Acids Research
Abstract
DNA can form diverse structures, which predefine their physiological functions. Besides duplexes that carry the genetic information, quadruplexes are the most well-studied DNA structures. In addition to their important roles in recombination, replication, transcription and translation, DNA quadruplexes have also been applied as diagnostic aptamers and antidisease therapeutics. Herein we further expand the sequence and structure complexity of DNA quadruplex by presenting a high-resolution crystal structure of DNA1 (5 -AGAGAGATGGGTGCGTT-3 ). This is the first quadruplex structure that contains all the internal A-, G-, C-, T-tetrads, A:T:A:T tetrads and bulged nucleotides in one single structure; as revealed by site-specific mutagenesis and biophysical studies, the central ATGGG motif plays important role in the quadruplex formation. Interestingly, our structure also provides great new insights into cation recognition, including the first-time reported Pb2+, by tetrad structures.
Instrument
J-815
Keywords
Circular dichroism, DNA structure, Biochemistry