In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications

August 27, 2020

Title

In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications

Author

Kyoung Suk Kang,# Hyun Uk Lee,# Moon Il Kim, So Young Park, Sung-Jin Chang, Ji-Ho Park, Yun Suk Huh, Jouhahn Lee, Mino Yang, Young-Chul Lee,corresponding author and Hyun Gyu Parkcorresponding author

Year

2015

Journal

Journal of Nanobiotechnology

Abstract

We have investigated the cytotoxic assay of Fe-aminoclay (FeAC) nanoparticles (NPs) and simultaneous imaging in HeLa cells by photoluminescent carbon nanodots (CD) conjugation. Non-cytotoxic, photostable, and CD NPs are conjugated with cationic FeAC NPs where CD NPs play a role in bio-imaging and FeAC NPs act as a substrate for CD conjugation and help to uptake of NPs into cancer cells due to positively charged surface of FeAC NPs in physiological media. As increase of CD-FeAC NPs loading in HeLa cell in vitro, it showed slight cytotoxicity at 1000 μg/mL but no cytotoxicity for normal cells up to concentration of 1000 μg/mL confirmed by two 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR) assays, with further observations by 4′,6-diamidino-2-phenylindole (DAPI) stained confocal microscopy images, possessing that CD-FeAC NPs can be used as potential drug delivery platforms in cancer cells with simultaneous imaging.

Instrument

FP-8500

Keywords

In-vitro cytotoxicity, Fe-aminoclay (FeAC), Carbon nanodots (CD), Cytotoxicity, Conjugation, Bio-imaging