Nitric oxide inhibitory constituents from the barks of Cinnamomum cassia
Shan He, Ke-Wu Zeng, Yong Jiang, Peng-Fei Tu
Six new compounds including one γ-butyrolactone, cinncassin A (1), two tetrahydrofuran derivatives, cinncassins B and C (2, 3), two lignans, cinncassins D and E (4, 5), and one phenylpropanol glucoside, cinnacassoside D (6), together with 14 known lignans (7 − 20) were isolated from the barks of Cinnamomum cassia. The structures of 1 − 6 were elucidated by extensive 1D and 2D NMR spectroscopic data analysis as well as chemical methods, and the absolute configurations were established by experimental and calculated ECD data. The anti-inflammatory activities of the isolates were evaluated on nitric oxide (NO) production in lipopolysaccharide (LPS)-induced BV-2 microglial cells. Compounds 5, 7, 8, and 15 showed potent inhibition activities with IC50 values of 17.6, 17.7, 18.7, and 17.5 μM, respectively.
Circular dichroism, Absolute configuration, Pharmaceutical