Title
Peptide oligomers from ultra-short peptides using sortase
Author
Natalya Voloshchuk, Long Chen, Qiang Li, Jun F. Liang
Year
2017
Journal
Biochemistry and Biophysics Reports
Abstract
Sortase A catalyzed ligation of ultra-short peptides leads to inter/intra-molecular transpeptidation to form either linear or cyclic oligomers dependent upon the peptide length. Cyclic peptides were the main products for peptides with more than 15aa. However, for ultra-short (<15aa) peptides, cyclic oligomers became predominant in prolonged reactions. Peptides with 1–3 aminoglycines were equally active but peptide oligomers from peptide containing more than one aminoglycine were prone to hydrolysis.
Instrument
J-710
Keywords
Circular dichroism, Secondary structure, Biochemistry