pH- and concentration-dependent supramolecular assembly of a fungal defensin plectasin variant into helical non-amyloid fibrils
Christin Pohl, Gregory Effantin, Eaazhisai Kandiah, Sebastian Meier, Guanghong Zeng, Werner Streicher, Dorotea Raventos Segura, Per H. Mygind, Dorthe Sandvang, Line Anker Nielsen, Günther H. J. Peters, Guy Schoehn, Christoph Mueller-Dieckmann, Allan Noergaard & Pernille Harris
Polypropylene fibres are difficult to dye using commonly used techniques due to the high crystallinity and non-polar aliphatic structure, that lack reactive places for dyes in the molecule. Dyeing PP fabric in scCO2 with antibacterial dyes merged the dyeing and finishing methods, resulting in a more productive technique in terms of water and energy consumption. Unmodified polypropylene fabric was dyed with 4-[2-[4-(ethenylsulphonyl)phenyl]diazenyl]-N,N-diethylbenzenamine antibacterial dye under scCO2 medium. The influences of scCO2 working parameters, such as dye concentration, pressure, dyeing time, and temperature, on fabric dye absorption expressed as color strength were studied. The color strength (K/S) was measured as well as CIELAB color parameters. The results were compared with its water dyeing analogue and it was observed that color strength as well as color depth (L) of the samples dyed in scCO2 were noticeably better than its water counterpart. In both scCO2 and water, the fastness properties (washing, rubbing, and light) of the dyed samples were excellent. Antibacterial activity of the dyed polypropylene sample in scCO2 was estimated and the results indicated good antibacterial efficiency.
protein, Self-assembly, fibril formation