Polyprenylated acylphloroglucinols as deubiquitinating protease USP7 inhibitors from Hypericum hookerianum
Qi-Qi Wang, Xiao-Dong Wang, Ling-Zhi Wu, Qiang-Qiang Fang, Ya-Nan Liu, Kun Jiang, Shi-Jin Qu, Chang-Heng Tan
Hookerianones A – E (1–5), five new polyprenylated acylphloroglucinols (PPAPs), along with six known ones, were isolated from the aerial parts of Hypericum hookerianum. Their structures were elucidated by analyses of MS, NMR, chiroptical properties, biogenetic pathway, and/or single crystal X-ray diffraction. A ubiquitin-rhodamine 110 assay showed that furohyperforin and hypercalin C, two representative PPAPs in this plant, inhibited more than 90% USP7 at the concentration of 10 μM.
Circular dichroism, Absolute configuration, Natural products, Medicinal, Pharmaceutical