Title
Protective Effects of Curcumin on Amyloid-β-Induced Neuronal Oxidative Damage
Author
Han-Chang Huang, Ping Chang, Xue-Ling Dai, Zhao-Feng Jiang
Year
2012
Journal
Neurochemical Research
Abstract
To investigate the protective effects of curcumin against amyloid-b (Ab)-induced neuronal damage. Primary rat cortical neurons were cultured with different treatments of Ab and curcumin. Neuronal morphologies, viability and damage were assessed. Neuronal oxidative stress was assessed, including extracellular hydrogen peroxide and intracellular reactive oxygen species. The abilities of curcumin to scavenge free radicals and to inhibit Ab aggregation and b-sheeted formation are further assessed and discussed. Curcumin preserves cell viability, which is decreased by Ab. The results of changed morphology, released Lactate dehydrogenases and cell viability assays indicate that curcumin protects Ab-induced neuronal damage. Curcumin depresses Ab-induced up-regulation of neuronal oxidative stress. The treatment sequence impacts the protective effect of curcumin on Ab-induced neuronal damage. Curcumin shows a more protective effect on neuronal oxidative damage when curcumin was added into cultured neurons not later than Ab, especially prior to Ab. The abilities of curcumin to scavenge free radicals and to inhibit the formation of b-sheeted aggregation are both beneficial to depress Ab-induced oxidative damage. Curcumin prevents neurons from Ab-induced oxidative damage, implying the therapeutic
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Ligand binding, Medicinal, Biochemistry