Title
Scale-up and inhibitory studies on productivity of lipase from Acinetobacter radioresistens PR8
Author
Krishna Kant Gupta, Anshul Nigam, Sharmili Jagtap, Ramadas Krishna
Year
2017
Journal
Journal of Bioscience and Bioengineering
Abstract
Acinetobacter radioresistens PR8 produces extracellular lipase depending upon growth media. In present work we not only screened the nutrient sources but also investigated the causes for variation in productivity. The nutrient sources investigated are, groundnut oil, groundnut cake and fresh groundnut. Lower lipase productivity was observed on fresh ground nut in contrast to groundnut oil and groundnut cake. The lipase productivity was examined in the batch and parameters monitored were bacterial growth, enzyme activity, pH, lipids and protein concentration. The aflatoxin B1 and oxalic acid present in fresh groundnut were found to be responsible for lower lipase productivity. The interaction studies of oxalic acid and purified lipase was confirmed with CD spectra analysis, isothermal titration calorimetry studies and fluorescence quenching. Therefore, the importance of economical cheap groundnut cake with no aflatoxin B1 and oxalates are proposed to be used for optimum lipase production.
Instrument
J-810
Keywords
Circular dichroism, Secondary structure, Ligand binding, Biochemistry