Seven new chemical constituents from the roots of Gentiana macrophylla pall
Shu-Bing Hou, Xin Wang, Rong Huang, Huan Liu, Hai-Min Hu, Wen-Yi Hu, Si-Tong Lv, Huan Zhao, Guang Chen
Gentimilegenins A, B (1, 2), (6R, 8R)-6-hydroxy swerimuslactone A (3), (6R, 8S)-6-hydroxy swerimuslactone A (4), 4-hydroxy roburic acid methyl ester (5), (±) 3′-hydroxy gentioxepine (6), N-heptacosanoyl anthranilic acid (7a), N-nonacosanoyl anthranilic acid (7b), together with 40 known compounds were isolated from the roots of Gentiana macrophylla Pall. Their structures were elucidated on the basis of comprehensive analysis of HRESIMS, IR, 1D-, 2D-NMR and X-ray diffraction. The anti-inflammatory effects of selected compounds were also evaluated through the detection of their inhibitory effects on NO production in LPS-induced RAW264.7 macrophage cells.
Circular dichroism, Absolute configuration, Stereochemistry, Natural products, Medicinal