Title
Seven new cytotoxic phenylspirodrimane derivatives from the endophytic fungus Stachybotrys chartarum
Author
Hong Zhang, Ming-Hua Yang, Fang-fang Zhuo, Na Gao, Xiao-Bei Cheng, Xiao-Bing Wang, Yue-Hu Pei, Ling-Yi Kong
Year
2019
Journal
RSC Advances
Abstract
Seven undescribed phenylspirodrimane derivatives, stachybochartins A–G (1–7), and four known analogues (8–11) were isolated from the endophytic fungus Stachybotrys chartarum obtained from Pinellia ternata. Stachybochartins A–D are four rare C–C-coupled dimeric derivatives and stachybochartin G features a seco-bisabosqual skeleton. Their structures and configurations were elucidated via spectroscopic analysis, electronic circular dichroism (ECD) calculations, the ECD exciton chirality method and the modified Mosher's method. Stachybochartins A–D and G displayed cytotoxic activities against MDA-MB-231 breast cancer cells and U-2OS osteosarcoma cells, with IC50 values ranging from 4.5 to 21.7 μM. Stachybochartins C and G exerted strong anti-proliferative activities against U-2OS cells in concentration- and time-dependent manners and induced apoptosis.
Instrument
J-810
Keywords
Circular dichroism, Absolute configuration, Cotton effect, Natural product, Pharmaceutical, Medicinal