Single Site Discrimination of Cytosine, 5‑Methylcytosine, and 5‑Hydroxymethylcytosine in Target DNA Using Anthracene-Tagged Fluorescent Probes
Jean-Louis H. A. Duprey, Gemma A. Bullen, Zheng-yun Zhao, Dario M. Bassani, Anna F. A. Peacock, John Wilkie, James H. R. Tucker
ACS Chemical Biology
The ability to discriminate between epigenetic variants in DNA is a necessary tool if we are to increase our understanding of the roles that they play in various biological processes and medical conditions. Herein, it is demonstrated how a simple two-step fluorescent probe assay can be used to differentiate all three major epigenetic variants of cytosine at a single locus site in a target strand of DNA.
Circular dichroism, Biochemistry