Solution structure of the 13th C2H2 Zinc Finger of Miz-1
Cynthia Tremblay, Mikaël Bédard, Marc-André Bonin, Pierre Lavigne
Biochemical and Biophysical Research Communications
Miz-1 is a BTB/POZ transcription factor that contains 13C2H2 Zinc Finger domains (ZF). Miz-1 transactivates and represses the transcription of a myriad of genes involved in many aspects of the biology of the cell. The detailed molecular interactions through which Miz-1 controls transcription, including its specific DNA binding via its ZF domains, remain to be understood and documented. In our effort to shed light into the structural biology of Miz-1, we have undertaken the determination of the structure of all its ZF and the characterization of their interactions with cognate DNA. The structure of ZF 1 to 10 have already been solved and characterized. Here, we present the structure of the synthetic Miz-1 ZF13 determined by 2D 1H-1H NMR.
Circular dichroism, Secondary structure, Ligand binding, Protein folding, Biochemistry