Structural studies of the protein endostatin in fusion with BAX BH3 death domain, a hybrid that present enhanced antitumoral activity
Rosa Maria Chura-Chambi, Helen Andrade Arcuri, Felipe Lino, Natan Versati, Mario Sergio Palma, Denize C. Favaro, Ligia Morganti
Biotechnology and Applied Biochemistry
Endostatin (ES) is an antiangiogenic protein that exhibit antitumor activity in animal models. However, the activity observed in animals was not observed in human clinical trials. ES-BAX is a fusion protein composed of two functional domains: ES, which presents specificity and is internalized by activated endothelial cells and the pro-apoptotic BH3 domain of the protein BAX, a peptide inductor of cellular death when internalized. We have previously shown (Chura-Chambi et al, Cell Death & Dis, v.5, e1371, 2014) that ES-BAX presents improved anti-tumor activity in relation to wild type ES. Secondary and tertiary structures of ES-BAX are similar to ES, as indicated by homology modeling studies and molecular dynamics simulations. Tryptophan intrinsic fluorescence and circular dichroism spectroscopy corroborate these data. 15N-HSQC NMR indicates that ES-BAX is structured but some ES residues have suffered chemical shift perturbations, suggesting that the BH3 peptide interacts with some parts of the ES protein. ES and ES-BAX present similar stability to thermal denaturation. The production of stable hybrid proteins can be a new approach to the development of therapeutic agents presenting specificity for tumoral endothelium and improved anti-tumor effect.
Circular dichroism, Protein denaturation, Secondary structure, Biochemistry