Surface plasmon resonance study of the interaction of N-methyl mesoporphyrin IX with G-quadruplex DNA

June 29, 2020

Title

Surface plasmon resonance study of the interaction of N-methyl mesoporphyrin IX with G-quadruplex DNA

Year

2020

Journal

Physical Chemistry Chemical Physics

Abstract

Surface plasmon resonance (SPR) was used to investigate the interaction between N-methyl mesoporphyrin IX (NMM) and different G-quadruplex (G4) topologies. The study was associated with circular dichroism analysis (CD) to assess the topology of the G4s when they interacted with NMM. We demonstrate the high selectivity of NMM for the parallel G4 structure with a dissociation constant at least ten times lower than those of other G4 topologies. We also confirm the ability of NMM to shift the G4 conformation from both the hybrid and antiparallel topologies toward the parallel structure.

Instrument

J-810

Keywords

Circular dichroism, G-quadruplex structure, Ligand binding, Chemical stability, Biochemistry