The application of G-quadruplex based assay with an iridium(III) complex to arsenic ion detection and its utilization in a microfluidic chip
Sheng Lin, Wanhe Wang, Chong Hu, Guanjun Yang, Chung-Nga Ko, Kangning Ren, Chung-Hang Leung, Dik-Lung Ma
Journal of Materials Chemistry B
In this work, the iridium(III) complex 1 was synthesized and employed to construct an assay which is based on G-quadruplex for detecting arsenic ions in aqueous solution. The assay achieved a detection limit of 7.6 nM (ca. 0.57 μg·L-1), and showed high selectivity towards arsenic ions over other metal ions. Additionally, the assay could function in natural water and a simple microfluidic chip was used to investigate the potential of this platform for real-time detection.
Circular dichroism, DNA structure, Ligand binding, Biochemistry, Materials