The chemical constituents of Gentiana macrophylla pall. under acidic condition using gentiopicroside-rich secoiridoids extract
Rong Huang, Wen-Yi Hu, Shu-Bing Hou, Huan Zhao, Xin Wang, Guang Chen
The chemical constituents of Gentiana macrophylla Pall. under acidic condition were explored using gentiopicroside-rich secoiridoids extract treated with hydrochloric acid. 2 new secoiridoid-like structures gentiagenin A (1) and B (2), together with 4 known compounds, swerimilegenin G (3), 4-hydroxyindan-1-one (4), erythrocentaurin (5) and swerimilegenin E (6) were identified using 1D, 2D NMR and HR-ESI-MS spectral technology. In the activity evaluation, the 2 new compounds, such as gentiagenin A (1) and B (2), exhibited stronger inhibitory effect on NO production in RAW264.7 macrophage cells than that of gentiopicroside.
Circular dichroism, Stereochemistry, Natural products