Title
Thiazole orange as a fluorescent probe: label-free and selective detection of silver ions based on the structural change of i-motif DNA at neutral pH
Author
Bei Hua Kang, Zhong Feng Gao, Na Li, Yan Shi, Nian Bing Li, Hong Qun Luo
Year
2016
Journal
Talanta
Abstract
Silver ions have been widely applied to many fields and have harmful effects on environments and human health. Herein, a label-free optical sensor for Ag+ detection is constructed based on thiazole orange (TO) as a fluorescent probe for the recognition of i-motif DNA structure change at neutral pH. Ag+ can fold a C-rich single stranded DNA sequence into i-motif DNA structure at neutral pH and that folding is reversible by chelation with cysteine (Cys). The DNA folding process can be indicated by the fluorescence change of TO, which is non-fluorescent in free molecule state and emits strong fluorescence after the incorporation with i-motif DNA. Thus, a rapid, sensitive, and selective method for the detection of Ag+ and Cys is developed with a detection limit of 17 and 280 nM, respectively. It is worth noting that the mechanism underlying the increase of the fluorescence of thiazole orange in the presence of i-motif structure is explained. Moreover, a fluorescent DNA logic gate is successfully designed based on the Ag+/Cys-mediated reversible fluorescence changes. The proposed detection strategy is label-free and economical. In addition, this system shows a great promise for i-motif/TO complex to analyze Ag+ in the real samples.
Instrument
J-810
Keywords
Circular dichroism, Protein folding, DNA structure, Ligand binding, Biochemistry, Materials