Understanding Secondary Structure
Proteins adopt unique conformations, optimized to perform specific
functions. Therefore, the information on the protein structure is
paramount to elucidating how proteins function.
Sampling Methods for Secondary Structure Estimation
| Method | FTIR Spectroscopy | Circular Dichroism Spectroscopy | X-Ray Crystallography |
|---|---|---|---|
| Sample Form | Solid and Liquid (Crystal and amorphous) | Liquid | Solid (crystal) |
| Concentration | 5 - 100% | 0.25 - 2.5% | 100% |
| Temperature Control | Yes | Yes | Yes |
| Auto Titration | No | Yes | No |
| Mapping | Yes | No | Yes |
| Secondary Structure Database | Under Study | Well-known | Standard Method |
| Information Collected | SSE | SSE, Tertiary Structure | SSE, Tertiary Structure |
Secondary Structure Estimation Using FTIR Spectroscopy
Infrared spectroscopy is an effective technique for component analysis and interaction analysis of biological samples such as proteins, sugars, and lipids. FTIR spectroscopy can be used to measure solid and liquid samples when applied to secondary structure analysis of proteins.
Secondary Structure Estimation Using Circular Dichroism