LC-4000 Series HPLC
The LC-4000 Series HPLC has key separation platforms at 50 MPa for conventional HPLC (3, 5, and 10 μm columns), 70 MPa for RHPLC (intermediate columns such as 2 to 3 μm and ‘coreshell‘), and 130 MP for UHPLC (sub 2 μm).
Advances in HPLC Solvent Delivery
For more than three decades JASCO analytical HPLC pumps have employed an asymmetric twin-piston solvent delivery system SSQD (Slow Suction, Quick Delivery) which provides significantly better flow and pressure characteristics than conventional twin-piston reciprocating designs. SSQD Technology offers the highest stability ‘compensated’ solvent delivery used in the PU-4180/85 RHPLC and PU-4280/85 UHPLC pump models, this makes it an excellent choice for detectors that are affected by flow pulsation like ECD, RI, and mass spec.
Pioneering Optical Design
As a pioneer in optical spectroscopy, JASCO has optical designs that few can match. We have developed a range of HPLC detectors with unrivaled performance, including the class-leading FP-4020 fluorescence detector with S/N greater than 2300:1 (Water Raman), RI-4035 UHPLC refractive index detector, and the unique CD-4095 circular dichroism detector for chiral chromatography.
HPLC Method Development
With the range of separation columns becoming increasingly diverse – the traditional 5μm fully porous particle is finally losing ground to some of the newer technologies such as the new superficially porous solid core (coreshell) and the wide variety of sub-2μm particles. Requirements for method development are becoming more demanding and time-consuming. For method development, the CO-4065 prep column oven can accommodate up to 10 analytical, preparative columns with automatic switching, and 6 or 10 solvent selection valves, controlled by the ChromNAV Method Development Module.
Compact and Easy to Use
The footprint of the LC-4000 Series HPLC is only 30 cm – requiring only a very small amount of bench space. For easy maintenance, all LC-4000 instruments feature front-panel access for replacing consumables such as pump seals, check valves, and detector light sources. The autosampler consumables such as the syringe parts are easy to access.
Front Panel Control
For users requiring front-panel control, the LC-4000 Series retains a keypad and backlit LED display. Keypad control can still be configured for convenient override when under PC control.
External Software Control
HPLC modules are often used for non-HPLC applications, such as high-accuracy feed pumps or stand-alone detectors for monitoring flow and reactors. For unattended operation RS-232 remains a convenient method for control and monitoring, using a simple communication protocol that can be included with each module.
Conventional Analytical HPLC and RHPLC Systems up to 70 MPa
The LC-4000 Series RHPLC system with a maximum operating pressure of 700 bar (70 MPa) has been designed to future-proof your HPLC requirements. The system can be used with conventional 3 – 5 μm particle size analytical columns at typical lower pressures around 10 to 20 MPa (1,500 to 3,000 psi) and can also be used with smaller particles such as shorter length UHPLC columns and Superficially Porous Particles (SPP) or Coreshell that require slightly higher pressure solvent delivery to provide optimum linear velocity through the column.
Analytical UHPLC Systems
The LC-4000 UHPLC System is great for chromatographers who need to run a large number of samples or get results quickly. Pioneering column technology continues to push particle sizes ever smaller. The LC-4000-130 UHPLC (130MPa – 20,000 psi) is designed to take advantage of the separation efficiency of the very small particles coupled with longer and narrower columns. The LC-4000-130 system incorporates many of the same features as the 70 MPa system, but with materials designed to withstand even higher pressures.