Webinar Series on Molecular Spectroscopy & Chromatography

Recorded Webinars

Watch previously recorded webinars and access presentation materials using the links below.

Excitation-Emission Matrices as fluorescence fingerprints of biofilms: A unique approach to assessing, remediating and troubleshooting water quality and biological treatment processes

In this previously recorded webinar, guest speaker Dr. Andrew Jacque discusses his innovative work to elucidate and remedy challenging water quality issues due to biofilm growth and other factors using a broad range of techniques including fluorescence and UV-Visible spectroscopy. Focus is on the insight provided by EEM fluorescence fingerprints.

Metabolic fingerprinting for diagnosis of fibromyalgia and other rheumatology disorders using Raman microscopy analysis of bloodspots

Guest speaker Dr. Luis Rodriguez-Saona, The Ohio State University, Food Science Department, shares his groundbreaking disease diagnosis work (FM, fibromyalgia) in this previously recorded webinar.

Applications in Fluorescence Spectroscopy:
Understanding, Analyzing & Optimizing Thermal Melts for Fluorescence, UV & CD Applications

This previously recorded seminar  takes a closer look at bio-analysis using temperature control and thermal melting.

Key points include:

  • Experimental design, including optimization of measurement parameters to achieve high quality data, such as
    • Single-point vs. spectral data
    • Staged temperature profiles for efficiency and accuracy
    • Ramping vs. equilibrating temperature
  • Analysis and data modeling, the pitfalls of poor data
  • Relevant examples will be provided to illustrate good practices and highlight what kind of information can be gained

Live Demonstration of Raman Imaging Microscopy

In this previously recorded demo of the NRS-4500 confocal Raman microscope, using a variety of different samples, we show:

  • How to use high sensitivity detection coupled with a high speed stage (QRI – quick Raman imaging) to perform large area fast imaging microscopy
  • Dealing with problem samples with either rough or tilted surfaces (SSI – in-focus measurement)
  • Finding particulate samples, automatically for faster set up and analysis (Simple Search)

This presentation is recommended for anyone interested to learn about Raman microscopy and how to get the best out of measurement for a range of sample matrices.

Fundamentals and Application of UV-Visible/NIR Spectroscopy: A Guide to Best Practices and Getting Good Data

This introductory webinar provides a review of UV-Visible theory and instrumentation basics, as well as a guide to best practices and getting good data, including information on:

  • How different instrument components effects measurement results
  • The difference between double beam and double monochromator instruments
  • How to correctly perform a baseline measurement
  • Integrating spheres and their applications

Live Demonstration of FTIR Imaging Microscopy

This demonstration is ideal for audiences interested in sampling and imaging techniques, good practice and optimization. We also introduce some unique features of the JASCO FTIR microscopy systems such as static mapping and wide area ATR imaging. This webinar covers:

  • IQ Mapping,
  • Fast Scan Mapping,
  • ATR Mapping and
  • Microscope interface software to create colored infrared maps.

Alternate Detection Techniques for HPLC

UV-Visible detection has been the standard for HPLC analysis for decades. This type of detection relies on a compound’s ability to absorb UV (or visible) light at certain wavelengths. But what about compounds that do not contain a chromaphore? Or compounds that have too low of a concentration to be detected via UV-Visible or where enantioselectivity is required? This webinar outlines alternate detection techniques such as fluorescence, refractive index, circular dichroism and evaporative light scattering to provide chemists with detection solutions outside of the UV-Visible realm.

FTIR Microscopy

This introductory webinar covers FTIR imaging. After a quick recap of FTIR theory and instrumentation, sample preparation, techniques and objectives are discussed. Additionally, hardware such as sample stages, detectors, and complimentary microscopy techniques are reviewed.

SFC Theory and Applications

This webinar covers the theory of supercritical fluids including their properties and advantages when applied to supercritical fluid chromatography (SFC). The design and requirements of the system hardware explains similarities to other chromatographic techniques like HPLC. Finally, a variety of industry applications is discussed highlighting the advantages of SFC for rapid analytical analyses and highly efficient preparative purifications.

Raman Microscopy and Imaging

This webinar takes a look into hardware and software necessary to obtain Raman images and mapping. Some of the applications reviewed include:

  • Mapping of pharmaceutical tablets
  • Identification and characterization of microplastics
  • Contamination of silicon wafer
  • Stress in semiconductor devices
  • Images of carbon materials

FTIR Theory, Instrumentation, and Techniques

In this webinar, Dr. James Burgess gives a presentation on FTIR Theory and Instrumentation, which includes an introduction to the theory of IR spectroscopy –  wavelength range, bond energies, and functional groups. A detailed introduction to the design concepts of the instrument follows. Finally, sampling techniques such as ATR (attenuated total reflectance), transmission, specular reflectance, and diffuse reflectance are discussed.

Circular Dichroism Measurement Optimization

Part 2 of our circular dichroism webinar series highlights sample considerations, measurement parameter optimization, and data processing functions including:

  • Selecting a buffer/solvent
  • The importance of monitoring the HT voltage
  • What is D.I.T.?
  • Optimizing the spectrum S/N
  • Subtracting a background spectrum
  • Converting to MRME

Vibrational Circular Dichroism

This webinar provides a review of vibrational circular dichroism, instrumentation and measurement parameters such as:

  • Types of sample that are amenable to vibrational circular dichroism
  • Effects of concentration and solvent selection
  • Measurement optimization and minimization of artifacts
  • Data analysis and comparison with ab initio calculation

Circular Dichroism Theory and Instrumentation

This introductory webinar provides a review of circular dichroism theory and instrumentation basics, as well as common biological applications including:

  • Structural characterization of proteins
  • VHH Antibody stability evaluation
  • Thermal stability studies
  • Structural characterization of nucleic acids
  • Microassay methods

Fluorescence Theory and Instrumentation

Whether working in a teaching, research, or industrial lab, getting high-quality, reproducible data – in which you have confidence – is essential. This introductory webinar provides a review of foundational fluorescence concepts and how to use this knowledge to optimize fluorescence. Theory and instrumentation for steady-state photoluminescence is discussed along with practical pointers for parameter selection to get the most out of your data.

Raman Theory and Instrumentation

This webinar begins with an introduction to the Raman Effect, including how visible light can give vibrational information. Raman instrumentation is thoroughly examined detailing function and options for lasers, gratings, and detectors. Finally, some time is given to imaging/sampling options common to Raman microspectrometers.