The high-throughput circular dichroism (HTCD) measurement system has been developed for use with either the J-1500 or J-1700 CD spectropolarimeter. This system can accommodate two 96-well plates (up to 192 samples) with protection at controlled temperature prior to measurement. Measurements using predetermined parameters for automated spectral scanning and/or temperature ramping measurement can be made with a Peltier thermostatted flow cell. In addition to CD, LD and absorbance data, fluorescence can be measured simultaneously (with optional accessory). Small sample amounts are required and can be recovered back to the micro-plate after measurement. The post-measurement analysis program allows batch processing of data and includes spectral correlation for quality control, determination of melting parameters and secondary structure estimation.
- Applications include peptides, proteins, nucleic acids, chiral organic components, monoclonal antibodies etc.
- Micro-plate temperature control
- Automated measurements with Spectra Manager-HTCD software; easily generated measurement sequences, including wavelength scan and temperature scan modes
- Automated data processing