The J-1100 Circular Dichroism spectrophotometer is a compact, general-purpose space-saving design. This simple yet powerful instrument is perfect for users looking to obtain more routine CD measurements in both industry and academia.
The J-1100 uses simple optimized parameters so that a wide range of users with different levels of CD knowledge can get right down to work and successfully obtain high-quality data. The wavelength range from 180 to 600 nm is suitable for obtaining secondary and tertiary structure measurements in the near- and far-UV. When fitted with a Peltier cell holder, the J-1100 be used for the measurement of CD, LD, and absorbance as a function of wavelength, temperature, and time.
The intuitive Spectra Manager™ Suite of spectroscopy software provides the user with sample measurement, independent from the data processing and analysis. This allows users to obtain new measurements while simultaneously analyzing previously acquired data for increased productivity.
Specifications
| Wavelength Range | RMS noise at 200 nm | Stray Light | Scan Speed | Wavelength Accuracy | Temperature Control | Cuvette Pathlengths | Detector |
|---|---|---|---|---|---|---|---|
| 180 – 600 nm UV to Visible | 0.03 mdeg High-sensitivity detection | < 0.0003% At 200 nm (NaI method) | Up to 5,000 nm/min Rapid spectral acquisition | ±0.2 nm Validated to USP/EP standards | -40 to 130°C With Peltier cell holder | 0.01–100 mm Multiple options available | High-sensitivity PMT |
Acquisition Modes
Wavelength scan
Collect full spectra across the UV-Visible range.
Temperature scan
Record full spectra or monitor multiple fixed wavelengths at set temperature intervals for melting studies.
Fixed-wavelength scan
Track CD signal at one or more wavelengths over time for kinetic denaturation or binding experiments.
Simultaneous CD + absorbance
Collect CD and HT (absorbance-proxy) data in a single scan. Fluorescence detection available via accessory.
Concentration-based analysis
Convert raw mdeg to molar ellipticity automatically within Spectra ManagerTM, JASCO’s integrated software.
Applications
Protein secondary structure
Quantify alpha-helix, beta-sheet, and random coil content. Ideal for biopharmaceutical comparability and lot-to-lot consistency.
Nucleic acid conformation
Distinguish B-, A-, and Z-DNA; characterize RNA folding, G-quadruplex formation, and protein-DNA binding interactions.
Chiral drug stereochemistry
Confirm enantiomeric identity and purity of small-molecule APIs. Supports regulatory submissions for chiral actives.
Thermal stability studies
Determine Tm and unfolding cooperatively for proteins, antibodies, and nucleic acids using the integrated Peltier system.
Enzyme kinetics
Monitor conformational changes in real time as substrates or inhibitors bind, complementing standard absorbance kinetics.
Teaching and core facility use
Compact footprint and intuitive Spectra ManagerTM interface make the J-1100 a practical option for multi-user core labs and course demonstrations.
Operational considerations
- Optional ozone free lamp
- For nitrogen free usage above 195 nm
- Nitrogen purge required
- Below 195 nm only
- Warm-up time
- ~30 min (lamp stabilization)
- Xenon lamp life
- ~1000 hours typical
- Footprint
- Compact benchtop design
- Software
- Spectra ManagerTM (Windows)
