Basic Theory of Spectroscopy
A basic overview of the various molecular spectroscopy techniques. JASCO covers topics such as Circular Dichroism, Raman Microscopy, Fluorescence, and Four-transform Infrared Spectroscopy.
Circular dichroism spectroscopy (CD) is an essential analytical technique used to analyze chirality in molecules through their optical activity.
UV-Visible/NIR spectroscopy can be divided into ultraviolet, visible, and near-infrared regions of the spectrum, depending on the wavelengths used. since its frequency is close to the overtone frequency of many natural vibrations, weak substance-specific absorption bands can be detected. It can therefore be used for non-destructive measurements, such as determining the sugar, lipid, protein content of foodstuffs and for identifying medicinals.
This technique offers a number of significant advantages over other spectroscopic or optical microscopy techniques, as it can be used for chemical or molecular analysis encompassing depth profiling and microscopic area mapping of samples with a spatial resolution of as little as 1 μm.
Fluorescence spectroscopy is routinely used for studying structural changes in conjugated systems, aromatic molecules, and rigid, planar compounds due to alterations in temperature, pH, ionic strength, solvent, and ligands.
Fourier-transform infrared spectroscopy is concerned with the vibration of molecules. Each functional group has its own discrete vibrational energy which can be used to identify a molecule through the combination of all of the functional groups.
FTIR is concerned with the vibration of molecules. Each functional group has its own discrete vibrational energy which can be used to identify a molecule through the combination of all of the functional groups. This makes FTIR microscopy ideal for sample ID, multilayer film characterization, and particle analysis.
The eBooks give a more in-depth look at the various molecular spectroscopy topics.