Increased Productivity of Chiral Separation in Semi-preparative Scale by SFC and Application to Structural Analysis of Fractionated Samples by CD Spectrometer | POSTER
August 20, 2025
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Enantiomers in chiral substances can be produced via methods like asymmetric synthesis, crystallization, enzymatic reactions, and optical resolution. Among these, HPLC with chiral stationary phases and, more recently, supercritical fluid chromatography (SFC) have been widely used, especially in pharmaceuticals. SFC offers faster separation and easier solvent removal due to the features of supercritical CO₂ evaporation. Analytical methods like NMR, XRD, ECD, and VCD help identify enantiomers.
In this study, bromuconazole was separated into four isomers using semi-prep SFC and compared with semi-prep LC. We applied fractionated peaks by semi-prep LC to ECD and VCD spectral measurements for determination of their absolute configurations.
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