Title
An electrochemical multienzymatic biosensor for determination of cholesterol
Author
Cristiana Bongiovanni, Tommaso Ferri, Alessandro Poscia, Maurizio Varalli, Roberto Santucci, Alessandro Desideri
Year
2001
Journal
Bioelectrochemistry
Abstract
This paper describes an electrochemical biosensor for free cholesterol monitoring. The sensor is a multienzymatic electrodic system in which horseradish peroxidase and cholesterol oxidase are simultaneously immobilized within a polymeric film, on the surface of a pyrolitic graphite electrode. From voltammetric and amperometric (flow-injection) data obtained, the efficiency, reproducibility and stability of the system are discussed. Results obtained, of interest for basic and applied biochemistry, represent a first step for construction of a mediator-free biosensor with potentialities for a successful application in the biosensor area.
Instrument
J-710
Keywords
Circular dichroism, Tertiary structure, Biochemistry