Color Analysis using a UV-Visible Spectrophotometer

About the Author

Leah Pandiscia received her PhD from Drexel University where she studied Biophysical Chemistry. She is a Spectroscopy Applications Scientist.

Introduction

Color is a visually perceived property that is derived from the reflected or transmitted spectrum of light interacting with the human eye. It is important for the development and production of color-based products to evaluate and obtain measurable factors that describe the color. JASCO has developed a variety of color analysis using a UV-Visible spectrophotometer for both reflectance and/or transmittance spectra of samples. The V-700 spectrophotometers have a double-beam optical system that provides high measurement accuracy and stability, which results in color evaluation that can be determined with high reproducibility.

**Figure 1.** Mechanism of color recognition by the human eye.

This application note tests the reproducibility of color evaluation made using JASCO’s color diagnosis system.

Experimental

Measurements were obtained for blue, red, and yellow pellets. Baseline measurement was performed using a standard white reference plate and then the sample was measured. Both reference and sample measurements were made with the specular component included. To confirm the measurement reproducibility 10 spectra were collected with the sample removed and replaced in between scans to assess the effects of changing the measurement position.

Keywords

040-UV-0044B, V-700, UV-Visible/NIR, Chromaticity, Color Analysis, Material, ISV-922 Integrating Sphere, Color Diagnosis program

Results

The reflectance spectra for each sample are shown in Figure 2. The figure on the right shows zoomed-in spectra in the wavelength range 740 – 780 nm. As shown in each spectrum, the difference between maxima and minima of the photometric value is less than 0.15%R, indicating high reproducibility measurements.

**Figure 2.** Reflectance spectra of the three colored pellets. The figures on the right are the zoomed in spectra from 740 to 780 nm.

The Color Diagnosis Program was used to calculate color coordinates from the measured reflectance spectra. Figure 3 shows the results plotted on XYZ, L*a*b*, Lab, and L*u*v* chromaticity diagrams. The calculated values are shown in Tables 1, 2 and 3 for the blue, red, and yellow pellets, respectively. For the color coordinates that represent chroma and hue (xy, a*b*, ab, u*v*), the difference between maxima and the minima are; x and y, less than 0.0012, a* and b*, less than 0.31, a and b, less than 0.18, u* and v*, less than 0.50. For the color coordinates that represent brightness (Y, L, L*), the difference between the maxima and minima is less than 0.1. All results indicate highly reproducible chromaticity values using the Color Diagnosis system.

**Figure 3.** Chromaticity diagrams for each color system and pellet.

Table 1. Color calculation results of blue color pellet.

	X	Y	Z	x	y	L*	a*	b*	L	a	b	u*	v*
1	23.77	23.94	65.84	0.2094	0.2108	56.03	4.58	-44.94	48.93	3.78	-50.19	-24.75	-70.73
2	23.77	23.94	65.81	0.2094	0.2109	56.03	4.57	-44.91	48.93	3.78	-50.15	-24.74	-70.69
3	23.74	23.91	65.70	0.2095	0.2109	55.99	4.57	-44.88	48.89	3.78	-50.09	-24.71	-70.62
4	23.76	23.93	65.72	0.2095	0.2110	56.01	4.57	-44.87	48.91	3.78	-50.08	-24.71	-70.60
5	23.76	23.92	65.71	0.2095	0.2110	56.01	4.56	-44.85	48.91	3.77	-50.06	-24.71	-70.58
6	23.79	23.96	65.85	0.2094	0.2109	56.04	4.57	-44.92	48.95	3.78	-50.16	-24.75	-70.70
7	23.80	23.97	65.85	0.2095	0.2110	56.06	4.55	-44.89	48.96	3.77	-50.12	-24.74	-70.65
8	23.81	23.98	65.83	0.2095	0.2111	56.07	4.55	-44.87	48.97	3.76	-50.08	-24.73	-70.62
9	23.80	23.97	65.79	0.2096	0.2111	56.06	4.55	-44.85	48.96	3.76	-50.06	-24.71	-70.58
10	23.80	23.97	65.81	0.2095	0.2111	56.06	4.55	-44.86	48.96	3.76	-50.08	-24.73	-70.61
Max-Min	0.07	0.07	0.15	0.0002	0.0003	0.07	0.03	0.09	0.07	0.02	0.13	0.05	0.14
Ave	23.78	23.95	65.79	0.21	0.21	56.04	4.56	-44.88	48.94	3.77	-50.11	-24.73	-70.64
SD	0.02289	0.02509	0.05879	0.00006	0.00008	0.0252	0.0104	0.0312	0.0256	0.0083	0.0456	0.0168	0.0512
CV%	0.10	0.10	0.09	0.03	0.04	0.4	0.23	-0.07	0.05	0.22	-0.09	-0.07	-0.07

Table 2. Color calculation results of red color pellet.

	X	Y	Z	x	y	L*	a*	b*	L	a	b	u*	v*
1	27.74	17.16	8.17	0.5226	0.3234	48.47	53.78	26.78	41.43	49.99	15.67	101.05	19.17
2	27.78	17.17	8.09	0.5238	0.3237	48.47	53.94	27.08	41.44	50.17	15.80	101.55	19.40
3	27.76	17.15	8.10	0.5236	0.3236	48.45	53.92	27.02	41.41	50.14	15.77	101.46	19.34
4	27.75	17.15	8.08	0.5238	0.3237	48.45	53.92	27.06	41.41	50.13	15.79	101.48	19.37
5	27.73	17.14	8.10	0.5236	0.3236	48.43	53.90	26.99	41.40	50.11	15.75	101.39	19.31
6	27.70	17.11	8.10	0.5235	0.3235	48.40	53.89	26.94	41.37	50.10	15.73	101.35	19.27
7	27.76	17.18	8.13	0.5231	0.3237	48.49	53.80	26.97	41.45	50.01	15.76	101.21	19.34
8	27.72	17.14	8.11	0.5233	0.3235	48.43	53.85	26.93	41.40	50.06	15.73	101.27	19.27
9	27.72	17.14	8.11	0.5233	0.3235	48.43	53.86	26.93	41.40	50.06	15.73	101.28	19.27
10	27.77	17.19	8.13	0.5231	0.3238	48.50	53.78	26.97	41.46	50.00	15.76	101.18	19.35
Max-Min	0.09	0.08	0.08	0.0012	0.0004	0.10	0.16	0.31	0.09	0.18	0.13	0.50	0.23
Ave	27.74	17.15	8.11	0.52	0.32	48.45	53.86	26.97	41.42	50.08	15.75	101.32	19.31
SD	0.0270	0.0230	0.0274	0.00038	0.00012	0.0288	0.0613	0.0852	0.0278	0.0640	0.0377	0.1545	0.0670
CV %	0.10	0.13	0.34	0.07	0.04	0.06	0.11	0.32	0.07	0.13	0.24	0.15	0.35

Table 3. Color calculation results of yellow color pellet.

	X	Y	Z	x	y	L*	a*	b*	L	a	b	u*	v*
1	27.74	17.16	8.17	0.5236	0.3234	48.47	53.78	26.78	41.43	49.99	15.67	101.05	19.17
2	27.78	17.17	8.09	0.5238	0.3237	48.47	53.94	27.08	41.44	50.17	15.80	101.55	19.40
3	27.76	17.15	8.10	0.5236	0.3236	48.45	53.92	27.02	41.41	50.14	15.77	101.46	19.34
4	27.75	17.15	8.08	0.5238	0.3237	48.45	53.92	27.06	41.41	50.13	15.79	101.48	19.37
5	27.73	17.14	8.10	0.5236	0.3236	48.43	53.90	26.99	41.40	50.11	15.75	101.39	19.31
6	27.70	17.11	8.10	0.5235	0.3235	48.40	53.89	26.94	41.37	50.10	15.73	101.35	19.27
7	27.76	17.18	8.13	0.5231	0.3237	48.49	53.80	26.97	41.45	50.01	15.76	101.21	19.34
8	27.72	17.14	8.11	0.5233	0.3235	48.43	53.85	26.93	41.40	50.06	15.73	101.27	19.27
9	27.72	17.14	8.11	0.5233	0.3235	48.43	53.86	26.93	41.40	50.06	15.73	101.28	19.27
10	27.77	17.19	8.13	0.5231	0.3238	48.50	53.78	26.97	41.46	50.00	15.76	101.18	19.35
Max-Min	0.09	0.08	0.08	0.0012	0.0004	0.10	0.16	0.31	0.09	0.18	0.13	0.50	0.23
Ave.	27.74	17.15	8.11	0.52	0.32	48.45	53.86	26.97	41.42	50.08	15.75	101.32	19.31
SD	0.0270	0.0230	0.0274	0.00038	0.00012	0.0288	0.0613	0.0852	0.0278	0.0640	0.0377	0.1545	0.0670
CV%	0.10	0.13	0.34	0.07	0.04	0.06	0.11	0.32	0.07	0.13	0.24	0.15	0.35

Analysis Conditions: Light Source, D65 (CIE 15:2004, JIS Z 8781-1:2012, ISO 11664-2:2007); Color-matching function, CIE 15:2004, JIS Z 8701:1982; Viewing angle, 2º; Data interval, 5 nm.

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

JASCO

Industry

Technique

Color Analysis using a UV-Visible Spectrophotometer

Introduction

Experimental

Keywords

Results

About the Author

Designed in Tokyo. TRUSTED globally.

Industry

Technique

Introduction

Experimental

Keywords

Results

Featured Products:

V-730 UV-Visible Spectrophotometer

V-750 UV-Visible Spectrophotometer

About the Author