UV-Visible

Double Beam UV-Visible Spectrophotometer

V-730

A general-purpose double beam UV-Visible spectrophotometer with a compact space-saving design and excellent spectroscopic performance great for routine bio, QC, teaching, and research applications.

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V-730 Double Beam UV-Visible Spectrophotometer

The V-730 UV-visible spectrophotometer is designed to be simple to use with precise results, with 1nm spectral bandwidth for high resolution and a linear dynamic range of over 3 AU across the entire spectrum.

A double-beam UV-Vis spectrophotometer splits the light source into two simultaneous beams: one through the sample, one through a reference. Absorbance is measured continuously against the reference, rather than requiring a separate blank run, giving the V-730 real-time baseline stability across long or repeated measurement sequences (0.0004 Abs/hour, measured after a one-hour warm-up at stable room temperature). Common applications include DNA and protein concentration (A260/A280), reaction kinetics, thermal melt analysis, color and turbidity testing, and routine QA/QC across pharmaceutical, industrial, and teaching laboratories.

Double Beam Optical Path - V-730

Innovative features include IQ Accessory automatic recognition and IQ Start for push-button operation of routine measurements. The V-730 covers a wavelength range of 190 to 1,100 nm with a fixed spectral bandwidth of 1.0 nm, supporting nucleic acid and protein quantitation, colorimetric assays, and materials QA/QC across the full range. Spectra Manager™ Suite*, the latest innovative, cross-platform spectroscopy software, or the iRM, a handheld controller with a color LCD touch screen, both provide full system control and advanced data processing.

*Spectra Manager™ CFR (for 21 CFR part 11 compliance) is available as an option.

V-730 UV-Visible Spectrophotometer — Double beam UV-Visible spectrophotometer

Compact Design

The V-730 is compact with dimensions of only 441 (W) x 468 (D) x 216 (H) mm.

Simplicity and Ease-of-Use

Both Spectra Manager™ and the iRM provide an intuitive interface allowing first-time users to operate the instrument with ease.

High-Speed Scanning

High-throughput optics and fast-response detectors allow the V-730 to scan at speeds of 10 to 8,000 nm/min without wavelength tracking errors, with a monochromator slew speed of up to 24,000 nm/min between measurement points. At maximum scan speed, a full 190 to 1,100 nm scan completes in approximately 7 seconds; a common QC range such as 350 to 900 nm completes in approximately 4 seconds, fast enough for routine dye verification, raw material identification, and time-sensitive reaction monitoring.

The V-730 does not rely on an instant-on xenon flash source. Illumination is provided by a halogen lamp for the visible range and a deuterium lamp for the UV range, with automatic switchover between 330 and 350 nm, delivering stable, low-noise illumination for accurate, repeatable measurements after a brief warm-up period.

Sampling Accessories

A wide range of sampling accessories including autosamplers & sippers, cell holders, flow cells, temperature controllers, and software applications.

Teaching and multi-user labs: An intuitive interface, either through a PC with Spectra Manager^™ Suite or standalone through the iRM color touchscreen controller, combined with IQ Accessory automatic recognition and fast scanning, helps new users get accurate results quickly in shared, high-turnover lab environments.

High-throughput and standalone QC: Optional 6-position Peltier cell changers (PSC-763, air-cooled, or NCP-705/706, water-thermostatted) and sippers enable rapid batch analysis without a dedicated PC, supporting routine QC workflows that run unattended.

The V-730 supports micro-volume sampling through an optional capillary adapter (minimum sample volume 3 µL), suited to nucleic acid and protein quantitation in bio and teaching labs. An optional temperature sensor accessory is available for monitoring sample temperature during the run.

Instrument Validation and Regulatory Compliance

The V-730 includes user-selectable validation to USP, EP, and JP (or manually editable), with optional IQ/OQ instrument qualification. For regulated environments, the optional Spectra Manager^™ CFR software module supports 21 CFR Part 11 workflows, including:

Username- and password-based access, with levels (Administrator, Power User, Limited User, User) assigned by a Workgroup Manager
Three-tier electronic signatures covering creation, review, and approval
An audit trail recorded in every data file, tracking all data processing and changes
A secure file structure that prevents deletion or overwrite of electronic records, with backup and restore

Highlighted Features

Double-beam spectrophotometer with single monochromator
Silicon photodiode detectors
Wide wavelength range – 190 to 1,100 nm
Fixed bandpass 1.0 nm for high resolution measurement
High-speed scanning up to 8,000 nm/min
Dedicated V-730 bio for bio-physical analysis such as kinetics, thermal melting, and protein/nucleic acid quant

V-730 FAQ’s

What Measurement Modes Are Available Out of the Box?

The V-730’s standard programs include an Abs/%T meter, quantitative analysis, spectrum measurement, fixed-wavelength measurement, time course measurement (including two-wavelength time course), plus built-in validation and daily maintenance routines, all run through Spectra Manager^™ Suite or the iRM controller. Dedicated bio-method templates for protein and nucleic acid quantitation and thermal melt analysis are available on the V-730BIO configuration, or on the standard V-730 with the optional micro-volume capillary adapter.

Can the System Operate Standalone via Touch Interface?

The V-730 can be controlled either way: through a PC running Spectra Manager^™ Suite software, or standalone through the iRM handheld controller’s color LCD touchscreen. Both provide full instrument control and data processing.

V-730 Quick Specs

Specification	V-730 Value
Wavelength range	190 to 1,100 nm
Spectral bandwidth	Fixed 1.0 nm
Photometric / dynamic range	–3 to 3 Abs
Scan speed	10 to 8,000 nm/min (slew speed 24,000 nm/min)
Stray light	0.02% (220–370 nm); 1% (198 nm)
Wavelength accuracy	±0.2 nm (at 656.1 nm)
Wavelength repeatability	±0.1 nm
Dimensions (W × D × H)	441 × 468 × 216 mm
Weight	15 kg
Power requirements	120VA
Compliance	USP, EP, JP validation; optional IQ/OQ; optional 21 CFR Part 11 (Spectra Manager CFR)

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JASCO

Double Beam UV-Visible Spectrophotometer

V-730

V-730 Double Beam UV-Visible Spectrophotometer