Ultra High-Speed Analysis of Cold and Sinus Medicines by UHPLC with Photodiode Array Detection
August 19, 2022
Introduction
Acetaminophen(Paracetamol), anhydrous caffeine, hesperidin, ethenzamide, tipepidinehibenzateor apronalide etc. are known as active ingredients of cold or sinus medicines. Acetaminophen has anti pyretic and analgesic effects. Anhydrous caffeine increases the analgesic effect. Hesperid in improves blood circulation and has ananti allergenic effect. Ethenzamidehas antipyretic, analgesicandantiphlogistic effects. Tipepidinehibenzatehas antitussive and expectorant effects. Apronalidehas analgesicand hypnotic effects.
The active ingredients contained in cold and sinus medicines were analyzed by UHPLC with PDA detection, which enables ultra-high speed data acquisition at the rate of 100 spectra/sec.
Experimental
Results
Figure 1 shows the chromatogram and contour plot of active ingredients contained in a cold and sinus medicine. Seven active ingredients and phenol added as internal standard(I.S.) are clearly separated within 1.5 min.
Figure 2 shows the on peak spectra of active ingredients contained in a cold and sinus medicine. Good spectrum was obtained for each component.
