Cadmium telluride nanocrystals as luminescent sensitizers in flow analysis
Paula R. Fortes, Christian Frigerio, Cristina I.C. Silvestre, João L.M. Santos, José L.F.C. Lima, Elias A.G. Zagatto
A fully automated multipumping flow system (MPFS) using water-soluble CdTe quantum dots (QD) as sensitizers is proposed for the chemiluminometric determination of the anti-diabetic drugs gliclazide and glipizide in pharmaceutical formulations. The nanocrystals acted as enhancers of the weak CL emission produced upon oxidation of sulphite by Ce(IV) in acidic medium, thus improving sensitivity and expanding the dynamical analytical concentration range. By interacting with the QD, the two analytes prevented their sensitizing effect yielding a chemiluminescence quenching of the Ce(IV)–SO32−CdTe QD system. The pulsed flow inherent to MPFS assured a fast and efficient mixing of all solutions inside the flow cell, circumventing the need for a reaction coil and facilitating the monitoring of the short-lived generated chemiluminescent species. QD crystal size, concentration and spectral region for measurement were investigated.
HPLC, CdTe, Quantum dots, Chemiluminescence, Multipumping flow system, Pharmaceutical formulations