Label-free , Optical Sensing of DNA-Ditryptophan Hybrids Supramolecular Assembly
Nidhi Gour, Jancy Nixon Abraham, Mohamed Chami, Axel Castillo, S. Verma
The Royal Society of Chemistry
Dichloromethane extra dry, N,N-dimethylformamide, sodium chloride, triethylamine were purchased from Acros Organics (Geel, Belgium). N,N′-dicyclohexylcarbodiimide, Nhydroxysuccinimide, sodium bicarbonate and dialysis tube (cut-off 2000 Mw) were supplied by Sigma Aldrich (Buchs, Swizerland). The ammonia solution 35% was purchased from Fisher Scientific SA (Wohlen, Switzerland). Fmoc-WW-OH was prepared by conventional solution phase synthesis. 5’CTCTCTCTCTTT-3’ modified at the 5’ end with an amine group through a hexyl spacer was purchased from Eurogentec (Belgium). WW-PEG was purchased from Genescript (Piscataway, New Jersey, USA). ESI mass spectra were recorded on 11HTS PAL -LC10A-API 150Ex. The analytical HPLC was equipped with a LC-Net II/ADC transmitter, a Jasco UV-2077 detector and a Jasco PU-980 pump.1H NMR spectra were recorded using a 300 –MHz Brucker NMR spectrophotometer in CDCl3 containing small amounts of TMS as internal standard.
HPLC, DNA, Ditryptophan Hybrids, Supramolecular Assembly