SIMULTANEOUS ESTIMATION OF LOSARTAN, HYDROCHLOROTHIAZIDE AND ATENOLOL FROM SOLID DOSAGE FORM BY RP-HPLC

April 23, 2020

Title

SIMULTANEOUS ESTIMATION OF LOSARTAN, HYDROCHLOROTHIAZIDE AND ATENOLOL FROM SOLID DOSAGE FORM BY RP-HPLC

Author

SAVITA S YADAV AND JANHAVI R. RAO

Year

2014

Journal

International Journal of Pharmacy and Pharmaceutical Sciences

Abstract

Objective: The objective of the present work was to develop and validate a simple, fast, precise, selective and accurate RP-HPLC method for thesimultaneous determination of Losartan, Hydrochlorothiazide and Atenolol from bulk and formulation. Methods: The separation of these three drugs was achieved on a Hypersil Gold column (250 mm X 4.6 mm, 5 µ) as stationary phase with a mobilephase consisting of methanol: water in the ratio of 95:5% v/v at a flow rate of 0.8 mL/min and UV detection at 225 nm. Results: The retention times were observed to be 2.633, 3.883 and 7.783 minutes for Losartan, Hydrochlorothiazide and Atenolol, respectively. Themethod was statistically validated for linearity, recovery, limit of detection, limit of quantification, accuracy and precision. Conclusion: The method was successfully applied for analysis of combined dose tablet formulation containing Losartan, Hydrochlorothiazide andAtenolol.

Instrument

HPLC

Keywords

HPLC, Atenolol, hydrochlorothiazide, Losartan