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Home / Applications / Analysis of Allantoin using HILIC HPLC

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Analysis of Allantoin using HILIC HPLC

By Masao Bounashita

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January 5, 2024

Introduction

Allantoin analysis
LC-2000 HPLC system

Allantoin is a urea hydantoin that has an anti-inflammatory effect as well as acting as cellular activator and therefore commonly used in pharmaceutical and cosmetic products. Allantoin is highly hydrophilic, and it is recommended that a column is used that works using a separation mechanism based on hydrophilic interaction chromatography (HILIC). The analysis of commercial eyedrops were measured as reported in this note.

Experimental

Instrument

PumpPU-2080
Vacuum degasserDG-2080-53
Column OvenCO-2060
AutosamplerAS-2057
Detector (UV-Visible)UV-2075

Conditions

ColumnFinePak SIL NH2-5
4.6mm x 250mm 5um
Mobile phase compositionAcetonitrile/Water 80:20
Flow rate1mL/min
Column temperature40 deg C
Injection volume10uL
Wavelength210nm
Standard sampleAllantoin 5ug/mL in mobile phase
Fig.1 Structural formula of allantoin.

 

Preparation

Dilute the eye-drops in mobile phase 10 times by volume, then filter using a  0.45um membrane filter.

Keywords

747012H

Results

Fig.2 Chromatogram of standard allantoin and sample of eyedrops
Fig.2 Chromatograms of a) Standard allantoin, b) Eyedrops
This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

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About the Author

Masao Bounashita

JASCO Application Note

Analysis of Allantoin using HILIC HPLC

Introduction

Allantoin analysis
LC-2000 HPLC system

Allantoin is a urea hydantoin that has an anti-inflammatory effect as well as acting as cellular activator and therefore commonly used in pharmaceutical and cosmetic products. Allantoin is highly hydrophilic, and it is recommended that a column is used that works using a separation mechanism based on hydrophilic interaction chromatography (HILIC). The analysis of commercial eyedrops were measured as reported in this note.

Experimental

Instrument

PumpPU-2080
Vacuum degasserDG-2080-53
Column OvenCO-2060
AutosamplerAS-2057
Detector (UV-Visible)UV-2075

Conditions

ColumnFinePak SIL NH2-5
4.6mm x 250mm 5um
Mobile phase compositionAcetonitrile/Water 80:20
Flow rate1mL/min
Column temperature40 deg C
Injection volume10uL
Wavelength210nm
Standard sampleAllantoin 5ug/mL in mobile phase
Fig.1 Structural formula of allantoin.

 

Preparation

Dilute the eye-drops in mobile phase 10 times by volume, then filter using a  0.45um membrane filter.

Keywords

747012H

Results

Fig.2 Chromatogram of standard allantoin and sample of eyedrops
Fig.2 Chromatograms of a) Standard allantoin, b) Eyedrops
This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
28600 Mary’s Court, Easton, MD 21601 USA • (800) 333-5272 • Fax: (410) 822-7526 • jascoinc.com/applications

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