![](https://jascoinc.com/wp-content/uploads/2024/06/BeStSel-secondary-structure-and-automatic-Y-axis.png)
THERMAL DENATURATION OF ANTIBODIES
Analyzed with BeStSel
![](https://jascoinc.com/wp-content/uploads/2024/06/Secondary-Structure-BeStSel-Application-Asset-5@2x.png)
Here, we compare Herceptin®, a well known breast cancer drug, and human IgG. The structure of the antibody at various temperatures was monitored by measuring the secondary structure CD spectrum from 30° to 85°C.
BeStSel offers fractional analysis of eight secondary structure elements, including three types of antiparallel β-sheet (Left-twisted, Right-twisted, Relaxed). Significant differences between Herceptin® and human-IgG in relaxed β-sheet change may reflect the formation of human IgG aggregates rather than simple denaturation as seen for Herceptin. BeStSel proves to be a powerful tool as this difference is clearly seen with the β-sheet model.
![](https://jascoinc.com/wp-content/uploads/2024/06/BeStSel-CD-Spectra-1440x420.png)
![](https://jascoinc.com/wp-content/uploads/2024/06/BeStSel-fractions-of-antibody-750x312.png)
Spectra Manager BeStSel utilizes the ‘BeStSel engine’, an algorithm developed by Dr. József Kardos and Dr. András Micsonai, et. al. at ELTE (Eötvös Loránd University, Hungary).