Analysis of Allantoin using HILIC HPLC
August 19, 2022
Introduction
Allantoin is a urea hydantoin that has an anti-inflammatory effect as well as acting as cellular activator and therefore commonly used in pharmaceutical and cosmetic products. Allantoin is highly hydrophilic, and it is recommended that a column is used that works using a separation mechanism based on hydrophilic interaction chromatography (HILIC). The analysis of commercial eyedrops were measured as reported in this note.
Experimental
Instrument
| Pump | PU-2080 |
| Vacuum degasser | DG-2080-53 |
| Column Oven | CO-2060 |
| Autosampler | AS-2057 |
| Detector (UV-Visible) | UV-2075 |
Conditions
| Column | FinePak SIL NH2-5 4.6mm x 250mm 5um |
| Mobile phase composition | Acetonitrile/Water 80:20 |
| Flow rate | 1mL/min |
| Column temperature | 40 deg C |
| Injection volume | 10uL |
| Wavelength | 210nm |
| Standard sample | Allantoin 5ug/mL in mobile phase |
Preparation
Dilute the eye-drops in mobile phase 10 times by volume, then filter using a 0.45um membrane filter.
Results
