Allantoin is a urea hydantoin that has an anti-inflammatory effect as well as acting as cellular activator and therefore commonly used in pharmaceutical and cosmetic products. Allantoin is highly hydrophilic, and it is recommended that a column is used that works using a separation mechanism based on hydrophilic interaction chromatography (HILIC). The analysis of commercial eyedrops were measured as reported in this note.
|Column||FinePak SIL NH2-5
4.6mm x 250mm 5um
|Mobile phase composition||Acetonitrile/Water 80:20|
|Column temperature||40 deg C|
|Standard sample||Allantoin 5ug/mL in mobile phase|
Dilute the eye-drops in mobile phase 10 times by volume, then filter using a 0.45um membrane filter.