Analysis of Allantoin using HILIC HPLC

By Masao Bounashita

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January 5, 2024

Introduction

Allantoin analysis
LC-2000 HPLC system

Allantoin is a urea hydantoin that has an anti-inflammatory effect as well as acting as cellular activator and therefore commonly used in pharmaceutical and cosmetic products. Allantoin is highly hydrophilic, and it is recommended that a column is used that works using a separation mechanism based on hydrophilic interaction chromatography (HILIC). The analysis of commercial eyedrops were measured as reported in this note.

Experimental

Instrument

PumpPU-2080
Vacuum degasserDG-2080-53
Column OvenCO-2060
AutosamplerAS-2057
Detector (UV-Visible)UV-2075

Conditions

ColumnFinePak SIL NH2-5
4.6mm x 250mm 5um
Mobile phase compositionAcetonitrile/Water 80:20
Flow rate1mL/min
Column temperature40 deg C
Injection volume10uL
Wavelength210nm
Standard sampleAllantoin 5ug/mL in mobile phase
Fig.1 Structural formula of allantoin.

 

Preparation

Dilute the eye-drops in mobile phase 10 times by volume, then filter using a  0.45um membrane filter.

Keywords

747012H

Results

Fig.2 Chromatogram of standard allantoin and sample of eyedrops
Fig.2 Chromatograms of a) Standard allantoin, b) Eyedrops
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About the Author

Masao Bounashita