Skip to content

JASCO JASCO

  • News
  • Events
  • E-Store
  • My Account
  • Contact Us
  • Worldwide
Search
Click to view menu
  • Products
    • Chromatography
      • HPLC
      • RHPLC
      • UHPLC
      • LC-MS
      • Preparative LC
      • Analytical SFC
      • Semi-Preparative SFC
      • Hybrid SFC
      • Fuel Analysis by SFC-FID
      • Preparative SFC
      • Supercritical Fluid Extraction
      • Chromatography Software
    • Molecular Spectroscopy
      • Circular Dichroism
      • High-Throughput CD
      • Vibrational CD
      • Circularly Polarized Luminescence (CPL)
      • Polarimeters
      • FTIR Spectrometers
      • FTIR Microscopy
      • FTIR Portable
      • Raman Microscopy
      • Palmtop Raman Spectrometer
      • Probe Raman
      • UV-Visible/NIR Spectrophotometers
      • UV-Visible/NIR Microscopy
      • Fluorescence Spectrophotometers
      • Film Thickness
      • Spectra Manager™ Suite
    • Refurbished
      • Refurbished HPLC Systems
      • HPLC Switching Valves
      • FTIR Accessories
  • Service
    • Service and Support Plans
    • Service Request Form
  • Applications
  • KnowledgeBase
  • Learning Center
    • Best Practice
      • Circular Dichroism Tips & Tricks for Biological Samples
      • CD Scale Calibration with ACS
      • Fluorescence Tips & Tricks
      • Raman Spectroscopy Tips & Tricks
    • Training Videos
      • ChromNAV
      • SF-NAV
      • Circular Dichroism
      • UV-Visible/NIR
      • Fluorescence
    • Training Seminars
      • Training Registration Form
    • Webinars
    • eBooks
    • Theory
      • Theory of Molecular Spectroscopy
      • Chromatography
  • About Us
    • President’s Message
    • Contact
    • History
    • Careers
  • News
  • Events
  • Worldwide
  • Shop
  • My Account
  • Contact Us

Home / Applications / Analysis of Nonionic Surfactants by HPLC-ELSD

  • Industry

  • Technique

Analysis of Nonionic Surfactants by HPLC-ELSD

By Keisuke Watanabe

PDF IconDownload This Application

August 19, 2022

Introduction

Surfactants are well known as a main constituent of commercial detergent and is widely used in various industrial and pharmaceutical fields due to various capabilities such as washing performance, moisture absorbency, osmosis, solubility, dispersibility, lubricity, antistatic ability, bactericidal property and anti-rust property. Nonionic surfactants have hydrophilic groups which are not ionized even when dissolved in water. Since it is hardly affected by water hardness and electrolytes, it can be used together with all other surfactants. Considering its nature, the usage of nonionic surfactants have increased dramatically and its affect on the environment has been pointed out. Since 2003, nonionic surfactants have been the test item for Water Quality Control Standards. This time, Triton X-100 and Polyethylene Glycol 400 as nonionic surfactant samples were measured and analyzed using an ELSD detector with Silica NH2 column in HILIC mode.

Experimental

Results

Figure 1 and figure 2 show the chromatograms of Triton X-100 and PEG400, respectively.

Figure 1 Chromatogram of Triton X-100.
Figure 2 Chromatogram of PEG400.
This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

Featured Products:

  • LC-4000 Series RHPLC

  • Tandem LC-MS

  • Single Quadrupole LC-MS

About the Author

Keisuke Watanabe

JASCO Application Note

Analysis of Nonionic Surfactants by HPLC-ELSD

Introduction

Surfactants are well known as a main constituent of commercial detergent and is widely used in various industrial and pharmaceutical fields due to various capabilities such as washing performance, moisture absorbency, osmosis, solubility, dispersibility, lubricity, antistatic ability, bactericidal property and anti-rust property. Nonionic surfactants have hydrophilic groups which are not ionized even when dissolved in water. Since it is hardly affected by water hardness and electrolytes, it can be used together with all other surfactants. Considering its nature, the usage of nonionic surfactants have increased dramatically and its affect on the environment has been pointed out. Since 2003, nonionic surfactants have been the test item for Water Quality Control Standards. This time, Triton X-100 and Polyethylene Glycol 400 as nonionic surfactant samples were measured and analyzed using an ELSD detector with Silica NH2 column in HILIC mode.

Experimental

Results

Figure 1 and figure 2 show the chromatograms of Triton X-100 and PEG400, respectively.

Figure 1 Chromatogram of Triton X-100.
Figure 2 Chromatogram of PEG400.
This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
28600 Mary’s Court, Easton, MD 21601 USA • (800) 333-5272 • Fax: (410) 822-7526 • jascoinc.com/applications

Close

Designed in Tokyo. TRUSTED globally.

View our support plans

Connect with JASCO

  • Facebook
  • Twitter
  • LinkedIn
  • JASCO Sales
  • 800-333-5272

Receive the latest promotions and special offers

  • This field is for validation purposes and should be left unchanged.
  • Careers
  • Press Kit
  • JASCO Privacy Policy
  • Sitemap
  • Environmental Policy

© , JASCO. All Rights Reserved.