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Home / Applications / High Resolution Separation of Hydrocarbons by Supercritical Fluid Chromatography

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High Resolution Separation of Hydrocarbons by Supercritical Fluid Chromatography

By Heather Haffner

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August 19, 2022

Introduction

It is fairly well known that SFC is used for fuel analysis of olefin determination in gasoline and non-aromatic and aromatic content in diesel among other fuel separations. In those applications the individual components within each class (non-aromatics, mono-aromatics, poly-aromatics) are not identified, but simply grouped to determine the percentage of each group. Recent interest in further separation and identification of the components within those compound classes has led to additional research.

Non-aromatic hydrocarbons are very difficult to retain as seen in the above mentioned fuel applications. In order to identify the components in the non-aromatics, a significant increase in retention will be required. Unique to SFC is the ability to join columns inline to increase the effective length of the separation column. Using two columns inline, the required retention was successfully achieved and the individual hydrocarbons were determined.

Experimental

Results

The chromatogram (Figure 1) of the non-aromatic hydrocarbon standard mixture is shown below. Besides C5-C9 which co-eluted, all of the hydrocarbons were successfully separated and identified. As the BP Calibration standard was diluted in hexane in order to have the FID signal on scale, the strong hexane signal likely contributed to the co-elution of C5-C9.

Figure 1. Chromatogram of the BP Calibration standard mixture of hydrocarbons (diluted in hexane before injection). The mixture contained C5-C10, C12,C14-C18, C20, C24, C28, C32, C36, and C40, all of which were identified.
This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

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About the Author

JASCO Application Note

High Resolution Separation of Hydrocarbons by Supercritical Fluid Chromatography

Introduction

It is fairly well known that SFC is used for fuel analysis of olefin determination in gasoline and non-aromatic and aromatic content in diesel among other fuel separations. In those applications the individual components within each class (non-aromatics, mono-aromatics, poly-aromatics) are not identified, but simply grouped to determine the percentage of each group. Recent interest in further separation and identification of the components within those compound classes has led to additional research.

Non-aromatic hydrocarbons are very difficult to retain as seen in the above mentioned fuel applications. In order to identify the components in the non-aromatics, a significant increase in retention will be required. Unique to SFC is the ability to join columns inline to increase the effective length of the separation column. Using two columns inline, the required retention was successfully achieved and the individual hydrocarbons were determined.

Experimental

Results

The chromatogram (Figure 1) of the non-aromatic hydrocarbon standard mixture is shown below. Besides C5-C9 which co-eluted, all of the hydrocarbons were successfully separated and identified. As the BP Calibration standard was diluted in hexane in order to have the FID signal on scale, the strong hexane signal likely contributed to the co-elution of C5-C9.

Figure 1. Chromatogram of the BP Calibration standard mixture of hydrocarbons (diluted in hexane before injection). The mixture contained C5-C10, C12,C14-C18, C20, C24, C28, C32, C36, and C40, all of which were identified.
This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
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