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Home / Applications / High Speed Analysis of Piperine in Pepper using UHPLC

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High Speed Analysis of Piperine in Pepper using UHPLC

By Satoe Iijima

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January 5, 2024

Introduction

Piperine is a particular spicy constituent that is contained in Piperaceae plants and is effective in improving energy metabolism, circulation of the blood and excessive sensitivity to cold.

It is also known that piperine has an antibacterial action, an antiseptic action and an insecticidal action, and it is contained more in black pepper. In this application, piperine in pepper was analyzed using Ultra High-performance Liquid Chromatography with Photodiode Array Detection.

Jasco XLC-3000

Experimental

Keywords

510002X

Results

Figure 1 shows the chromatogram and the contour plot of the piperine standard sample. A longer method analysis time was used than typical for UHPLC taking into consideration that the actual sample may require separation of other components.

Figure 1. Chromatogram and contour plot of a piperine standard sample
1: Piperine

Figure 2 shows a spectrum of piperine.

Figure 2. Spectrum of piperine sample

Figure 3 shows the chromatogram and the contour plot of the extract of coarsely ground pepper after extraction using a Supercritical Fluid Extraction system.

Figure 3. Chromatogram and contour plot of coarsely ground pepper
1: Piperine

Sample Preparation

Coarsely ground pepper was extracted using a Supercritical Fluid Extraction system and its extract was dissolved in 1 mL of Acetonitrile and diluted with Acetonitrile to 1/100 concentration. Then it was filtrated using 0.2 mm membrane filter.

Supercritical Fluid Extraction Conditions

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.

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About the Author

Ms Iijima is a member of the chromatography separations team at JASCO Corporation main applications laboratory in Tokyo Japan.

JASCO Application Note

High Speed Analysis of Piperine in Pepper using UHPLC

Introduction

Piperine is a particular spicy constituent that is contained in Piperaceae plants and is effective in improving energy metabolism, circulation of the blood and excessive sensitivity to cold.

It is also known that piperine has an antibacterial action, an antiseptic action and an insecticidal action, and it is contained more in black pepper. In this application, piperine in pepper was analyzed using Ultra High-performance Liquid Chromatography with Photodiode Array Detection.

Jasco XLC-3000

Experimental

Results

Figure 1 shows the chromatogram and the contour plot of the piperine standard sample. A longer method analysis time was used than typical for UHPLC taking into consideration that the actual sample may require separation of other components.

Figure 1. Chromatogram and contour plot of a piperine standard sample
1: Piperine

Figure 2 shows a spectrum of piperine.

Figure 2. Spectrum of piperine sample

Figure 3 shows the chromatogram and the contour plot of the extract of coarsely ground pepper after extraction using a Supercritical Fluid Extraction system.

Figure 3. Chromatogram and contour plot of coarsely ground pepper
1: Piperine

Sample Preparation

Coarsely ground pepper was extracted using a Supercritical Fluid Extraction system and its extract was dissolved in 1 mL of Acetonitrile and diluted with Acetonitrile to 1/100 concentration. Then it was filtrated using 0.2 mm membrane filter.

Supercritical Fluid Extraction Conditions

Keywords

510002X

This document has been prepared based on information available at the time of publication and is subject to revision without notice. Although the contents are checked with the utmost care, we do not guarantee their accuracy or completeness. JASCO Corporation assumes no responsibility or liability for any loss or damage incurred as a result of the use of any information contained in this document. Copyright and other intellectual property rights in this document remain the property of JASCO Corporation. Please do not attempt to copy, modify, redistribute, or sell etc. in whole or in part without prior written permission.
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