High Speed Analysis of Piperine in Pepper using UHPLC

Download PDF August 18, 2022


Piperine is a particular spicy constituent that is contained in Piperaceae plants and is effective in improving energy metabolism, circulation of the blood and excessive sensitivity to cold.

It is also known that piperine has an antibacterial action, an antiseptic action and an insecticidal action, and it is contained more in black pepper. In this application, piperine in pepper was analyzed using Ultra High-performance Liquid Chromatography with Photodiode Array Detection.

Jasco XLC-3000



Figure 1 shows the chromatogram and the contour plot of the piperine standard sample. A longer method analysis time was used than typical for UHPLC taking into consideration that the actual sample may require separation of other components.

Figure 1. Chromatogram and contour plot of a piperine standard sample
1: Piperine

Figure 2 shows a spectrum of piperine.

Figure 2. Spectrum of piperine sample

Figure 3 shows the chromatogram and the contour plot of the extract of coarsely ground pepper after extraction using a Supercritical Fluid Extraction system.

Figure 3. Chromatogram and contour plot of coarsely ground pepper
1: Piperine

Sample Preparation

Coarsely ground pepper was extracted using a Supercritical Fluid Extraction system and its extract was dissolved in 1 mL of Acetonitrile and diluted with Acetonitrile to 1/100 concentration. Then it was filtrated using 0.2 mm membrane filter.

Supercritical Fluid Extraction Conditions

About the Author

Ms Iijima is a member of the chromatography separations team at JASCO Corporation main applications laboratory in Tokyo Japan.